Walt Disney a Short Biography Essay Example

Walt Disney: a Short Biography Essay Walt Disney: A Short Biography Walt Disney was a man with a vision. I have chosen Walt Disney because of his contribution to the history of the United States of America. He is a legend, and a hero to some. The Disney name has come to represent creation, optimism, and creation. Walt completely changed the film industry and a television pioneer, and he helped the country create a much greater appreciation for family morals and values. Walt Disney was born in Chicago Illinois to his parents Elias and Flora Disney on December 5th 1901. Walt was one of five children. Right after Walt was born the family moved to Missouri to the town of Marceline. Walt lived most of his childhood in this town. At a very young age had an interest in art, he would spend hours drawing and sketching, and would even try to make a little money by selling his artwork to neighbors. When he reached 15 he started to pursue his art career by attending McKinley High school in Chicago where he studied photography and art. Times were always a little tough for the Disney family. Walt’s father was a very stern man, and since there was often little money made matters even worse at times. Although times were always a little tough Walt’s mother always encouraged Walt and his brother Roy to pursue their talents as artists. At the age of 16 Walt tried to enlist in the military, he was rejected because he was underage, but that did not stop Walt. Walt then joined the Red Cross and was then sent overseas to France. Walt spent a year in France chauffeuring around Red Cross officials helping the war effort and also driving an ambulance. We will write a custom essay sample on Walt Disney: a Short Biography specifically for you for only $16.38 $13.9/page Order now We will write a custom essay sample on Walt Disney: a Short Biography specifically for you FOR ONLY $16.38 $13.9/page Hire Writer We will write a custom essay sample on Walt Disney: a Short Biography specifically for you FOR ONLY $16.38 $13.9/page Hire Writer The ambulance that Walt drove was covered from front to back in cartoons that Walt had drew and sketched. After returning to the United States from France, Walt began to pursue a career in commercial art. He started a small company by the name of â€Å"Laugh-O-Grams† which failed. He then traveled to Hollywood with 20 dollars and a suitcase full of clothes to try to start again. Once in Hollywood he had a break with his â€Å"Alice Comedies†, and soon became a recognized figure in Hollywood, soon after Walt married a woman by the name of Lillian Bounds in 1925. Lillian was one of Walt’s first employees and soon after they had two daughters by the names of Diane and Sharon. In 1932 Walt created a production called â€Å"Flowers and Trees† which was the first ever color cartoon and won Walt’s studio its first academy award. He released another color cartoon in 1937 called â€Å"The Old Mill† which was the first short cartoon to utilize the â€Å"multi-plane camera technique†. Later that year Walt released â€Å"Snow White and the Seven Dwarfs† which was the first ever full length musical animated movie. The movie was a huge success and earned a staggering $1, 499, 000 dollars in the middle of the depression. Today snow white is still considered one of the greatest movies of all time because of how it has lasted throughout the years. During the next five years Walt Disney Studios released other classics such as Pinocchio, Fantasia, Dumbo, and Bambi. With the success of Walt Disney Studios, Walt had another dream, and that dream was to create a clean, family friendly and organized amusement park. His dream soon became true when Disneyland opened its doors in the summer of 1955. Walt also became a television pioneer with television production starting in 1954 and was the one of the first to offer color programming with the â€Å"Wonderful World of Color† which aired in 1961. Walt Disney was a true American pioneer and success. A man that would not accept failure as an option, embraced the American tradition brought the future to us with his films. His legacy lives on till this day and will most likely continue on into the future.

All About the Chapultepec Castle in Mexico City

All About the Chapultepec Castle in Mexico City Located in the heart of Mexico City, Chapultepec Castle is a historic site and local landmark. Inhabited since the days of the Aztec Empire, Chapultepec Hill offers a commanding view of the sprawling city. The fortress was the home of legendary Mexican leaders including Emperor Maximilian and Porfirio Diaz and played an important role in the Mexican-American War. Today, the castle is home to the first-rate National Museum of History. Chapultepec Hill Chapultepec means â€Å"Hill of the Grasshoppers† in Nahuatl, the language of the Aztecs. The site of the castle was an important landmark to the Aztecs who inhabited Tenochtitlan, the ancient city which would later become  known as Mexico City. The hill was located on an island in Lake Texcoco where the Mexica people made their home. According to legend, the other people of the region did not care for the Mexica and sent them to the island, then known for dangerous insects and animals, but the Mexica ate these pests and made the island their own. After the Spanish conquest of the Aztec Empire, the Spanish drained Lake Texcoco to control flooding issues. On the grounds near the castle, at the base of the hill in the park near the  Nià ±os Heroes  monument, there are ancient glyphs carved into the stone during the reign of the Aztecs. One of the rulers mentioned is Montezuma II.   The Castle After the fall of the Aztecs in 1521, the hill was largely left alone. A Spanish viceroy, Bernardo de Glvez, ordered a home built there in 1785, but he left and the place was eventually auctioned off. The hill and assorted structures upon it eventually became the property of the municipality of Mexico City. In 1833, the new nation of Mexico decided to create a military academy there. Many of the older structures of the castle date from this time. Mexican-American War and the Hero Children In 1846, the Mexican-American War began. In 1847, the Americans approached Mexico City from the east. Chapultepec was fortified and placed under the command of General Nicolas Bravo, a former president of the Mexican republic. On September 13, 1847, the Americans needed to take the castle to proceed, they did, then secured the fortress. According to legend, six young cadets remained at their posts to fight off the invaders. One of them, Juan Escutia, wrapped himself in the Mexican flag and leaped to his death from the castle walls, denying the invaders the honor of removing the flag from the castle. These six young men are immortalized as the Nià ±os Heroes or â€Å"Hero Children† of the war. According to modern historians, the story is likely embellished, but the fact remains that Mexican cadets did defend the castle bravely during the Siege of Chapultepec. The Age of Maximilian In 1864, Maximilian of Austria, a young European Prince of the Habsburg line, became emperor of Mexico. Although he spoke no Spanish, he was approached by Mexican and French agents who believed that a stable monarchy would be the best thing for Mexico. Maximilian resided at Chapultepec Castle, which he had modernized and rebuilt according to the European standards of luxury at the time with marble floors and fine furniture. Maximilian also ordered the construction of Paseo de la Reforma, which connects Chapultepec Castle to the National Palace in the center of town. Maximilian’s rule lasted three years until he was captured and executed by forces loyal to  Benito Juarez, the president of Mexico, who maintained he was the legitimate head of Mexico during Maximilians reign. Residence for Presidents In 1876, Porfirio Diaz came to power in Mexico. He took Chapultepec Castle as his official residence. Like Maximilian, Diaz ordered changes and additions to the castle. Many items from his time are still in the castle, including his bed and the desk from which he signed his resignation as president in 1911. During the Mexican Revolution, various presidents used the castle as an official residence, including Francisco I. Madero, Venustiano Carranza, and Alvaro Obregà ³n. Following the war, Presidents Plutarco Elias Calles and Abelardo Rodriguez resided there. The Castle Today In 1939, President Lazaro Cardenas del Rio declared that Chapultepec Castle would become the home of Mexicos National History Museum. The museum and castle are a popular tourist destination. Many of the upper floors and gardens have been restored to look as they did during the age of Emperor Maximilian or President Porfirio Diaz, including original beds, furniture, paintings, and Maximilians fancy coach. Also, the exterior is renovated and includes the busts of Charlemagne and Napoleon that had been commissioned by Maximilian. Near the entrance to the castle is a massive monument to the fallen during the 1846 Mexican-American War, a monument to the 201st Air Squadron, a Mexican air unit which fought on the side of the Allies during World War II  and old water cisterns, a nod to Lake Texcocos former glory. Museum Features The National Museum of History includes pre-Colombian artifacts and displays about ancient cultures of Mexico. Other sections detail important parts of Mexican history, such as the war for independence and the Mexican Revolution. Oddly, there is little information about the 1847 Siege of Chapultepec. There are numerous paintings in the museum, including famous portraits of historical figures such as Miguel Hidalgo and Josà © Marà ­a Morelos. The best paintings are the masterpiece murals by legendary artists Juan O’Gorman, Jorge Gonzlez Camarena, Jose Clemente Orozco, and David Siqueiros.

Basic requirements of a written exam Essay Example | Topics and Well Written Essays - 750 words

Basic requirements of a written exam - Essay Example According to Bloom’s taxonomy for levels of understanding, six levels include knowledge, comprehension, application, analysis, synthesis, and evaluation; typically, for the first three levels, written examinations are most suitable and can be made cost-effective where many students are to be evaluated. Nevertheless, written exams need to be set appropriately to ensure that they deliver a reliable evaluation of students’ knowledge. This is usually achievable through use of multiple-choice questions as they enable teachers to pinpoint depth of knowledge concerning detail. Coupled with proper setting, written examinations also require a defined grading system (Asklund and Bendix 1). For a written exam to be effective, it should be valid, reliable and consistent, transparent, fair, efficient and ease in handling, and allow for useful feedback. Validity refers to measuring what is actually intended, where results achieved should be accounted for practically. Reliability and consistency enable achievement of similar results regardless of number of assessors and over time; more so, with marking schemes and assessment criterion. Transparency implies that the written examinations should test in such a way that the stated learning objectives are clearly met. Fairness calls for all students to be subjected to the same form of assessment and as per the criterion set. Lastly, to avoid written examinations being perceived as burdensome, they should be allocated sufficient time for both being done and marking, and afterwards, students should have their progress communicated to them in due course. However, the requirements of a written exam differ with the type it is that is, whether unseen, revealed, open book, multiple choice or take home assignments. An unseen written exam occurs where the content of the exam are unknown to students whereas, in a revealed exam, students are made aware of the

The financial pro's and con's of outsourcing - how to judge wether an Essay

The financial pro's and con's of outsourcing - how to judge wether an activity should be outsourced - Essay Example Beneifer Irani states that outsourcing offers freedom to pass on non – core, yet important sectors of its administration on companies that specializ in those very individual aspects. (Beneifer Irani)2 The advantage of outsourcing lies in the fact that it helps companies to cut costs and stay ahead in the competition. Indeed Companies can save up on operational costs to half by outsourcing. They get access to cheaper and more efficient labour and save up on training costs. They can also access better technologies at lower cost. Outsourcing also helps to increase productivity as they pay to a third party purely on output. It allows the company to concentrate on its core competency. Companies today want to make use of the outsourcing advantage in order to progress and stay abreast of the competition. The main disadvantage of outsourcing is that the company can get into serious trouble if the service provider is unable to provide the business or service due to bankruptcy, lack of funds, labour troubles etc. There is also a loss of control over the process that is outsourced and there may be a loss of quality. Unless the outsourced service is exclusively provided to one company, there is chance of the service provider failing one or the other principal and this can cause disruption in smooth flow of work. Outsourcing jobs to offshore destinations, is causing some unemployment as both high and low-end jobs are moving out. There is often a conflict between companies, national economies and individuals and it is difficult to reconcile all the stakeholders. The company must consider outsourcing on the level of its core requirements. If an activity is considered a core activity then it should not outsource it to a third party as it is not likely to be handled by better people than the company has. The very fact that it is a core competency declares that the company is most competent to perform it. However core competency has separate meaning for

Green communication Assignment Example | Topics and Well Written Essays - 500 words

Green communication - Assignment Example thers, thus saving on the resources (which are primarily power and cooling costs at the data center) that would have been required to maintain all the servers. Moreover, virtualization will reduce the operating and capital costs for the university, while still delivering high application ability. The implementation of solid state disks (SSD) is also a path for going green in data storage. Since SSDs are fixed and do not have movable parts, they are not susceptible to damage or shock like the traditional hard drive. Moreover, they offer cost savings since they have a lower energy productivity and higher efficiency. The university can also employ the use of massive array of idle disks, abbreviated as MAIDS. MAIDS are advantageous over the traditional storage methods in that they tend to have a relatively larger storage capacity over their counterparts. Furthermore, they have lower power consumption than tapes and hard drives. All these attributes point to saving on resources, which is the key to a green environment. A green data center can be defined as the repository management, dissemination and storage of data in which the systems (lighting, mechanical and electrical) are designed for minimum environmental impact and maximum energy efficiency (Greentechmedia.com, 2015). Green data centers are environmental conscious have lower carbon footprints and operate at lower power levels, hence reducing both capital and operating cost for an organization. The university can ensure that the data center is environmental friendly by minimizing power constraints. This can be achieved by locating the servers to areas that power is cheaper through server virtualization. Furthermore, the university can monitor the individual power consumption of the devices that it has and determine what should be changed (Technet.microsoft.com, 2015). Green data centers should have efficient lighting, like LEDs, that can reduce the power consumption by 80%. The cooling system should be as

The development of the low cost airline industry in asia

The development of the low cost airline industry in asia 1. Introduction This report will address the assignment topic. ‘Discuss the development of the low cost airline industry in Asia. The topic chosen for this report is the development of Low cost airline in Asia Tourism industry. To address the complexities of this topic, it is necessary to begin the report with an understanding of low cost airline and Aisa tourism industry. In a broader area, it is necessary to concern the impacts of Aisa, and implication for the future. It is important to keep in mind the global impact on the Tourism Industry that these changes could have. However, base on the limitation of time and information, this report will focus on China, Hong Kong, and Japan to investigate all of the issuse of low cost airline.Moreover, because these three regions are most popular to discuss and people will familiar with the situations of these regions.Therefore, the following content will investigate these three regions and all of the issues. 2. What is Low Cost Airline? According to ITF (2002), the operation model of low cost carrier is point to pint services, usually low cost carriers will mainly focus on short haul flight between 400 -600 nautical miles, even they will not have catering and entertainment service inflight. Moreover, they will use one aircaft type to enhance the utilisation and reduce the maintanace cost; also they will often using secondary airports with cheaper landing charges. In addition, they will focus on the lower income market and base on the time of booking and choice of flight 3. The Developmet of Low Cost Airine Refer to Thomas Bieger, Andreas Wittmer (2006), in the early 1970s, the first Low cost airline mode was operated by Southwest Airlines in the United Stated. For the charateristics of this mode, the Southwest Airlines was just providing the single class and no frills air travel services that means they will no provide the meal and snacks, and just provide the economic class for passengers. Moreover, the fare price was low and high restriction, that means the fare will have a high penality for those who need to change the time and location of the fare. Base on the liberalisation, deregulation and release of open skies policy. More Low cost airline was inccur and base on the growth of air travel (WTO 2002), the demand of aircraft was huge. Therefore, air travel was a potental market to develop. For the development of Asia, In 1998 the two low-cost carriers, Skymark Airlines and Air Do, entered, respectively, the Tokyo-Fukuoka route and the Tokyo-Sapporo route. In South Korea, the LCCs, Hansung Airlines and Jeju Air, entered the domestic market in 2005 and 2006 respectively, and two more LCCs, namely, Yeongnam Air and Jin Air, just entered the market in July 2008. In Hong Kong and Macau, the two Special Administrative Regions of China, Oasis Hong Kong and Viva Macau are the respective LCCs, both serving long-haul international routes in effect, established in 2004 Viva Macau is the first long-haul LCC in Asia In 2005, the first low costairline was established in China which was Spring airline. Spring Airline was established by Spring Travel and their operation model was quite similar to Southwest Airliness, they also just provide single class and no frills services. Spring Airine was just provide the domestic flight and have about 34 routes within China. 4. Feasibility Study of Potential Market According to World Airline Report (2008), In 2008 the Revenue passenger kilometreswas about 355,243. For 2009 the Revenue passenger kilometres was about 313,469.Although, the revenue was decrease about 11.8% (Figure, 2). However, it was huge revenue for the airline idustry within Asia Pacific. On the othe hand, in 2009 the amount of passengers was abot 75,363,000 and in 2008 the amount of passengers was about 84,586,000 (Figure, 2), and decrease about 10.9%. However, it can prove that the Asian market still have a large demand of the aviation industry. 3. 1 China Base on the World Tourism Organisation Report (2004), About 108 million foreign visitors travelled to China, and 18 percent increase from 2003 and a 10 percent increase from 2002.Foreign tourists who stayed at least overnight reached 41 million, up 24 percent from 2003 and 11 percent from 2002. Moreover, foreign exchange revenue from tourism reached 25 billion US dollars, a 47 percent increase from2003 and a 25 percent increase from 2002. In addition, the number of domestic tourists will reach 930 million.Domestic tourism revenue isexpected to exceed 400 billion Yuan, 5.9 percenthigher than that of 2003 and 3.1 percent higher than that of 2002. 3.2 Hong Kong On the other hand, in 2004, Hong Kong obtained over 21.8 million tourist arrivals, representing an increase of 40.4% as compared to 2003.Moreover,the strong performance of the tourism industry was mainly attributable to the spectacular growth of the Mainland arrivals, especially after the implementation and continual expansion of the Individual Visit Scheme which covers residents of Beijing, Shanghai Guangzhou, under the auspicesof the Close Economic Partnership Agreement(CEPA) with Mainland China. During 2004, over 12.2 million Mainland tourists visited Hong Kong,with over 4.2 million travelling under the Individual Visit Scheme. Meanwhile, other major overseas markets also exceeded pre-SARS level andare growing healthily. 3.3 Japan According to World Tourism Organisation Report (JNTO) international visitors to Japan in 2004 (January to December) is estimated to be 6.1million tourists, up 17.9% from last year. Base on World Tourism Organisation Report (2004), the total number of Japanese travellers to Republic of Korea in 2004 was 2.4 million tourists and it exceeded the same figure in 2002. This is attributed to the popularity of Korean TV dramasinJapan.The total number of Japanese travellers to China in 2004 (January to November) was 3 million. The total number of Japanese travellers who went to Taiwan, province of China and Singapore was 890,000 and 598,000 respectively. Both figures exceeded 2003 figures when SARS was an issue, but did not reach the figure of 2002. 4. Low Cost Carrier Contribution in China According to Civil Aviation Administration of China Report, In 2007, GDP reached 24.6619 trillion yuan, an increase of 11.4%, the fifth consecutive year growth rate of more than 10%. Base on the CAAC report, over the last five years Chinese civil aviation has seen a rapid growth, averaging 20 % annual growth rate. In 2007, the completion of the total Transportation turnover 36.53 billion ton-km, passenger turnover 279.17billion person- kilometers, passenger transport volume of 190 million passengers, cargo and Mail turnover 11.64 billion tonkm, cargo and mail transport volume of 4.019 million tons, respectively, compared with 19.5% last year17.8%, 16.3%, 23.5%, and 15.0% (Figure 1,3) Low cost airline to complete a total turnover of 1.85 billion ton-km, an increase of 216.9 percent, accounting for 5.1% of the industry, an increase of 3.2 percentage points (Figure 2) 4.1 Low Cost Carrier Contribution in Hong Kong By the Census and Statistics Department report (2008), In the first quarter of 2008, Hong Kongs GNP increased by 6.5% over a year earlier to $427.3 billion at current market prices. The Gross Domestic Product (GDP), estimated at $409.3 billion at current market prices in the same quarter, recorded a 9.6% increase during the period. Compared with GDP, the value of Hong Kongs GNP was larger by $18.0 billion in the first quarter of 2008, representing a net external factor income inflow of the same amount, and equivalent to 4.4% of GDP in that quarter. Moreover, Hotel occupancy across all categories of hotels in January 2009 was 79%, eight percentage points lower than in January 2009. The geographic locations with the highest occupancy were Yau Ma Tei/Mong Kok, where hotels recorded average occupancy rate of 85%. The average achieved hotel room rate across all hotel categories was HK$1,117, 10.3% lower than in January 2008. 4.3 Low Cost Carrier Contribution of Japan According to Department of National Accounts Economic and Social Research Institute Cabinet Office, the report of Quarterly Estimates of GDP (2009), the GDP offirst quarterly was about 521,560 trillion yen, and in 2008 the GDP of first quarterly was about 569,139 trillion yen. Moreover, for the private consumption between 2008 and 2009, the amount of privateConsumption in 2008 was about 312, 069 and in 2009 was about 303,639 trillion yen. 5The Drawbacks of Low Cost Carrires Base on the analysis of China, Hong Kong, and Japan, although low cost carrires canIncrease the econmoic level of these three regions. However, for the econonmic point of view, when the Gross Domestic Product getting increase, the cost of living will also getting increase. Becase of the inflation problem for the goods and products, people may not affordable to purchase the goods and products. Morover, it will also occurthe unequal income distribution, that means rich guy can get more rich and poor guy will get more poor.On the hand, when the demand of low cost airline increase, that mean the demand of air traffic will increase too. For this issue, it may occur the overcrowding for the airport. The capacity of the airport a limit, if too many flight come to the airport, it may occur the congestion of air traffic, the congestion of runway and apron and increase the accident rate of air traffic. Moreover, air traffic accounts in a sizeable way in the emissions of carbon dioxide the most credited international estimates go from a minimum of 3% to a maximum of 10%. However, Aviation accounts for 2% of man-made global carbon emissions, and with current global demand for the aviation industry increasing, it is predicted to grow to 3% by 2026. 6. Conclusion To sum up this report, after the analysis of the development of low cost Airline in Asia, all of the evidents and data can prove that low cost Airline a continue contribute the Asian regions such as China, Hong Kong, and Japan. Airline Industry is one of the key transports within Asia, the government of these regions, need to pay atttention to develop and control. But for the further development of Low cost airlines within Asia, there are some barrires will affect the expansion of Low cost carriers. The first one is the deregulation issued, China and Japan are more restrict their sky policy such as Chinese government will restirct the route of the flight and the price stucture of the air ticket, because the government will more protect the local airlines. Second, China and Japan are lack of secondary airport. For China areas, all of the major airports are main airport. If low cost carrires want to entry this market, they can not redcue the operation cost and can not compete with the regional airlines. Thrid, Hong Kong aviation market was monopoly by Cathy Pacify; the Oasis Hong Kong was a good example to prove that if the market was monpoly by a large airlineCompany, they can not survive. The last one is the fuel cost issue, although the international crude oil prices have recently fallen from the peak of US$70-odd to US$50-odd. However, the fuel cost is one of the major cost of airline companies. If the crude oil prices return to peak of US$70-odd or higher, the lost cost airline can not survive with this issue. Therefore, for the further development of Low cost carrires these kinds of issue are necessary to concern and may to have greater negotiation of all the countries to discuss all these issues.

P-type ATPases Essay example -- Chemical Biology, Biochemistry

Describe the mechanism of P-type ATPases; select one P-type ATPase and examine how its physiological role has been investigated. The P-type ATPases are a large family of membrane enzymes, with 476 different subtypes categorised in the Swiss-Prot protein information database. The P-type or E1-E2 ATPases were first discovered and categorised by Jens Christian Skou, a Danish physician-turned-physiologist in 1957. Skou discovered the Na+/K+ ATPase, and later shared half of the 1997 Nobel Prize for Chemistry in reward for his work (Skou 1997). As mentioned above, the P-type ATPases have a broad range of interventions, for example, the use of the Digitalis toxin in the treatment of heart failure (Rang et al. 2007). There are 5 broad classes of P-type ATPase, and numerable sub-classes. Human ATP-ases are primarily grouped in classed I, II and IV, with group III consisting of bacterial enzymes, and group V largely unclassified (Stokes and Green 2003). An especially important role of an ATPase in humans is the transport of sodium and potassium ions across the cell membrane. It is this Na+/K+ ATPase that J.C Skou discovered, and worked on for most of his academic career (Skou 1997). The fundamental basis of the P-type ATPase's ability to function is its capacity to form 2 conformational states, E1 and E2. Both of these states are ion-binding, one allowing intramembrane ion binding, and the other with an extramembrane ion binding site. The Na+/K+ ATPase is an anti-porter, transporting Na+ ions out of the cell, and K+ ions into the cell, at a 3:2 ratio (Na:K), against the concentration gradient (Lehninger et al. 2000). The process of transporting ions across the membrane is a 4 step mechanism, as shown in figure 1. Firstly, 3 N... ... a Participant in the Active Transport of Sodium and Potassium in the Human Erythrocyte. Journal of Biological Chemistry 235(6), pp. 1796-1802. Rang, H. P. et al. 2007. Rang and Dale's pharmacology. 6th ed. Edinburgh: Churchill Livingstone/Elsevier, pp. xiii, 829 p. Shull, G. E. et al. 1985. Amino-acid sequence of the catalytic subunit of the (Na+ + K+)ATPase deduced from a complementary DNA. Nature 316(6030), pp. 691-695. Skou, J. C. 1997. The Nobel Prizes 1997. Stockholm: Nobel Foundation. Skou, J. C. 2004. The Identification of the Sodium Pump. Bioscience Reports 24(4), pp. 436-451. Skou, J. C. and Esmann, M. 1992. The Na,K-ATPase. Journal of Bioenergetics and Biomembranes 24(3), pp. 249-261. Stokes, D. L. and Green, N. M. 2003. STRUCTURE AND FUNCTION OF THE CALCIUM PUMP. Annual Review of Biophysics and Biomolecular Structure 32(1), pp. 445-468.